Journal: Proceedings of the National Academy of Sciences of the United States of America
Article Title: HIF2α negatively regulates MYCN protein levels and promotes a low-risk noradrenergic phenotype in neuroblastoma
doi: 10.1073/pnas.2516922122
Figure Lengend Snippet: EPAS1 expression is associated with the chromaffin cell lineage and low-risk neuroblastoma. ( A – D ) Visualization of EPAS1 ( A ), DDC ( B ), and SLC18A1 ( C ) expression in UMAP plot of the developing human adrenal medulla 7 to 17 PCW ( D ), dataset from Jansky et al. . ( E ) In neuroblastoma tumors EPAS1 expression is positively correlated with low-risk tumors lacking MYCN amplification. ( F ) Expression of DDC and EPAS1 are positively correlated in neuroblastoma tumors. ( G ) DDC expression is correlated to increased overall survival. ( H ) Expression of SLC18A1 and EPAS1 are positively correlated in neuroblastoma tumors. ( I ) SLC18A1 expression is correlated to increased overall survival. ( J ) Mapping of EPAS1 expression in tSNE of neuroblastoma single cell nuclei show enrichment in endothelial cells, and in noradrenergic low-risk tumor cells, dataset from Bedoya-Reina et al. . ( K ) DDC expression is enriched for in noradrenergic low-risk tumor cells. ( L ) SLC18A1 expression is enriched for in noradrenergic low-risk tumor cells. ( M ) Violin plots depicting median expression of the indicated genes in each group. Groups in red font represent noradrenergic neuroblastoma and groups in blue font represent undifferentiated high-risk neuroblastoma. *** Indicates FDR < 0.01.
Article Snippet: Human umbilical vein endothelial cells (HUVEC; ATCC CRL-4053) were propagated under identical incubation conditions in Endothelial Cell Growth Medium MV2 (PromoCell, C-22022) supplemented with 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin.
Techniques: Expressing, Amplification
ATCC is a verified supplier 
ATCC manufactures this product 
